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The spindle assembly checkpoint (SAC) has evolved to ensure the fidelity of sister chromatid segregation. It has two main functions: prevention of chromosome-to-chromatid (prevention) and sister chromatid (sister) crossover (crossover) during chromosome segregation. Recent studies have shown that the mitotic checkpoint complex (MCC) is one of the key molecules involved in the SAC and shares a complex with kinetochores during early mitosis in both budding and fission yeast. Our previous study in Xenopus embryos demonstrated that the MCC prevented sister chromatid crossover and its function had a more direct relationship with the SAC than that in budding yeast and fission yeast. In this study, we showed that the MCC, which is composed of Mad1, Mad2, Bub1 and Bub3, was the first factor to be recruited to the kinetochores in early mitosis, and that Mad1 was required for MCC formation. The MCC then prevented anaphase entry and chromosome-to-chromatid, but not sister, crossover. Moreover, we found that the Nedd1 (an E3 ubiquitin ligase)-Bub3 complex was required for the SAC in Xenopus embryos. These results suggest that the MCC functions as a bridge between the SAC and metaphase-anaphase transition and also have novel and functionally distinct roles in Xenopus early mitosis.The United States has announced the planned release of the John McCain trial documents, and the stunning news that the media in the United States has chosen not to pay any attention to the McCain news.

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Sarah is currently a Technical Program Manager at the NASA Jet Propulsion Laboratory. Her job is to help manage and direct the Lab’s participation in the Cassini mission. She has also worked previously as a Mars Pathfinder Program Manager for JPL. I had the opportunity to interview Sarah and asked her the following questions.

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