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Staphylococci are distributed worldwide. The epidemiology of these microorganisms among pets and livestock is poorly known in Europe. The aim of this study was to investigate the presence of Staphylococcus aureus isolates in clinical samples of cattle and control dogs in the north of Portugal. A total of 150 samples were collected https://www.ctworkers.org/slot88-maxwin/ from skin lesions, milk samples, nasal swabs and blood samples from cattle and the control dog population during 2008. The specimens were cultured on mannitol salt agar and staphylococci were isolated and identified by tube agglutination test and tube coagulase activity. Eighty-six staphylococci were isolated from cattle and control dogs, being 62% of the bovine isolates and 70% of the canine isolates classified as S. aureus. The most frequent isolation was from nasal swabs (42%) of the control dogs and (36.2%) of the cattle. A higher percentage of S. aureus was found in nasal swabs (40.4%) than in other sampled sites (range 22.2-23.5%). The majority of bovine and canine isolates harbored the PVL genes. Fifty-six and 63% of the canine and bovine isolates, respectively, harbored the mupA gene. Twenty-eight STs were detected among bovine and canine S. aureus isolates, most of them with multiple locus variable tandem repeat analysis (MLVA). Ten STs were identified only in isolates from control dogs, including three novel STs (ST188, ST501
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