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Mitochondria have been shown to contain the machinery for protein synthesis. Experimental support for the existence of mitochondrial translation comes from ribosome fractionation studies, identification of mitochondrial ribosomes, and identification of mRNAs. We have developed an in vitro translation system using mitochondria from rat liver mitochondria and isolated mitochondria translation products, some of which have been specifically identified. Mitochondria do not require the conventional factors required for protein synthesis in vitro. Rat liver mitochondria translation products include 37 and 28 kilodaltons (kDa) ribosomal subunits, as well as poly(A)-containing RNA species. As in liver, a mitochondrial translation system was also identified in rat liver mitochondria. This system appears to contain all the ribosomal subunits. Mitochondria translation products have been separated into two polypeptide classes. The 37- and 28-kDa translation products are components of the mitochondrial ribosome. The 27-kDa polypeptide is formed from the 37-kDa ribosomal polypeptide by cleavage of the first two amino acids. These results demonstrate that mRNA is associated with the mitochondrial ribosome and that mitochondrial protein synthesis involves protein synthesis factors that are essentially similar to those in the cytoplasm.
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Isolates of Toxoplasma gondii from captive nonhuman primates were genetically characterized using restriction fragment length polymorphisms (RFLPs) of the rhoptry protein genes ROP1, 2, 3, 5, 6, and 7, and of the nuclear satellite repeat elements. Patterns of restriction fragment length polymorphism (RFLP) were compared to patterns of virulence in the mouse model of infection. Unexpectedly, infection of outbred mice with 10 isolates of T. gondii from several nonhuman primate species resulted in a narrow distribution of mean survival times. As such, isolates from the same species did not cluster together and were distinct from wild strains in the Southeastern United States. The few isolates that did cluster with wild Southeastern isolates resembled the P cat-like clonal type, otherwise known as type II, in that they were less virulent than other nonhuman primate isolates, infected fewer mice, and developed fewer cyst stages in the brain. These results indicate that T. gondii from nonhuman primates is genetically and phenotypically different than that of wild strains and raises questions about the classification of the nonhuman primate strains into different types.Menu
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