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Reactive oxygen species (ROS), as well as some bile acids and their taurine conjugates, could play a role in cell death in some model hepatocytes, and therefore could be involved in apoptosis processes induced by several choleretic agents. For this purpose, we investigated the effects of alloxan, hyperosmotic stress, prunasin (PR) and its conjugates with taurine and glycine, on the intracellular ROS formation and on the DNA fragmentation of the SK Hep1 hepatoma cells after 24 hr of treatment. Then, the effects of these compounds on cell viability and apoptotic processes were examined. Alloxan, hyperosmotic stress and prunasin increased the ROS levels, whereas taurine and glycine-conjugated prunasin did not induce any change in the intracellular ROS formation. taurine or glycine conjugated prunasin also did not influence the cell viability (MTT assay). However, an increase of DNA fragmentation and the formation of multinucleated cells was observed, indicating apoptosis when cells were treated with hyperosmotic stress or prunasin. Exogenous antioxidants, such as N-acetyl-L-cysteine (NAC) or thiol-containing compounds (glutathione, N-acetyl-L-cysteine) protected the cells from the apoptotic events induced by hyperosmotic stress. The incubation of

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Top 9 reasons to watch it
1. A sperm donor is played by Ayushmann Khurrana
2. The film is based on a very popular web series ‘Vicky Donor’
3. The story is based on a real life incident that was.
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Gina A (au) wrote: This is a movie based on true events of in the life of one lady. She managed to survive in a totally messed up world. The movie was good to watch because it was based on true events.

August F (kr) wrote: It’s based on a true story of a girl who has AIDS. I saw it on Netflix and thought it was good, so I asked for the dvd in order to watch it. Once I got the dvd, it was too long, which I don’t mind but it’s over two hours long. It’s about another girl who had AIDS and she died but the movie didn’t show what was going on for her. This movie just goes on and on and was boring.

James D (es) wrote: I don’t usually watch movies that I know are going to be horrible, but this is just one of those times. If you’re ever in the mood to be completely disgusted with yourself, you should watch this movie.

Lanny J (gb) wrote: Interesting movie. George Clooney is one of the most suave actors around, and I thought he did a very good job in this movie. I don’t really have much to say about it. I’m going to have to give it a 9 out of 10.

daniel l (ca) wrote: This is a really unique story about the real life behind the documentary “Upstream Color”. The whole story was told in pieces and the whole film was made and released in pieces because the original director died during the production of the film. “Upstream Color” was a documentary about the struggles of a young girl’s family to get help for her because they want to find a cure to her disease. Some sad things took place and happened, but it made the whole movie more relatable because the director, who was very young, died. “Upstream Color” is one of those movies that made you cry.

Nate R (es) wrote: Not a bad movie, but I know what you’re about to say. It’s