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Dubspot describes the low-end as being nothing more than a “collection of. 27 Sep 2017. FREE! Download and save the Wave’s Complete.RTAS-TDM-VST7-1.1.6.ZA.REPACK.ccD”aac in 2.68.@bib14] or in a pseudo-intracellular space, which forms a branched meshwork structure inside the cell ([@bib10]). If one assumes a simple flux of multivesicular bodies in and out of the lysosome, the observed influx and efflux of autophagosome lumens with vesicular structures indicates that the majority of intraluminal vesicles/vesicles and vesiculogenomes are formed inside the lysosomes. Formation of these vesicles inside the lysosomes could be restricted to the perinuclear space. This might help to explain the observed enrichment of multivesicular bodies at the perinuclear space ([@bib36]). Our analysis of the vesicular structures in dynamic optical sections throughout cell death supports this hypothesis.

The ability of autophagosomes to deliver their content to the lysosomes depends on the fusion of autophagosomes with lysosomes. The only other example of fusion of autophagosome with any cellular component is the fusion of autophagosomes with ER membranes, which is also an important process for the degradation of ER cargo ([@bib16; @bib41]). A study on the dynamic behavior of autophagosomes during mitosis showed that lysosomal activity is most strongly reduced after mitosis but remains relatively constant through the G1 phase of the next cell cycle. Fusion of autophagosomes with the ER could result in their redistribution into the perinuclear space ([@bib8]). This statement is in agreement with the results of our live cell imaging study ([Fig. S2](#app2){ref-type=”sec”}). During mitosis there was a pronounced increase in the number of autophagic structures in the perinuclear region of the cell as well as a decrease in the number of autophagosomes in the peripheral cytoplasm. This suggested that autophagosomes fuse with the ER before mitosis. During the G1 phase of the next cell cycle

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