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Version: This time it happened on Windows 7. We are having trouble with web applications,. Folder 552-1979, Agricultural Extension. Average milk yield (lb. J. 53. 64. Fat test (%)..Ligand-induced conformational change of human gonadotropin subunit beta.
It is known that LH and FSH both consist of heterodimer and heterotrimer. In both homologous and heterologous hormone pairings, subunit interactions, disulfide bonds, and glycosylation of subunits are the major factors that determine hormone activities. In this study, we determined the conformational changes of the LH beta-subunit in the assembly of LH and FSH, as well as the effects of deglycosylation of alpha- and beta-subunits on the hormone activities. Conformational changes of LH beta-subunit were analyzed by limited proteolysis of the recombinant human FSH beta-subunit (hFSH beta) with trypsin or PNGase F. We then examined the effect of these changes on the hormone activities of the LH and FSH. Deglycosylation of the LH beta-subunit significantly increased the hormone activities of LH and FSH. It appears that the conformational change in the LH beta-subunit caused by carbohydrate removal makes the conformation of the protein more similar to that of the FSH beta-subunit, resulting in more conformational stability of the homodimer. Moreover, the conversion of the hormone activity from LH to FSH was due to the conformational change of the LH beta-subunit but not of the FSH beta-subunit. Our results provide important information in the regulation of gonadotropin activities. provides for a type
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